Review





Similar Products

rabbit polyclonal anti p fak tyr 397  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology rabbit polyclonal anti p fak tyr 397
Rabbit Polyclonal Anti P Fak Tyr 397, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p fak tyr 397/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
rabbit polyclonal anti p fak tyr 397 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
rabbit anti tyr 397 fak  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti tyr 397 fak
Rabbit Anti Tyr 397 Fak, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti tyr 397 fak/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
rabbit anti tyr 397 fak - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
tyr 397  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc tyr 397
Tyr 397, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tyr 397/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
tyr 397 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
rabbit polyclonal antibody against phospho fak tyr 397  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit polyclonal antibody against phospho fak tyr 397
Rabbit Polyclonal Antibody Against Phospho Fak Tyr 397, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibody against phospho fak tyr 397/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
rabbit polyclonal antibody against phospho fak tyr 397 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
rabbit anti phospho fak tyr 397  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti phospho fak tyr 397
Rabbit Anti Phospho Fak Tyr 397, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti phospho fak tyr 397/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
rabbit anti phospho fak tyr 397 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
pfak tyr 397  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc pfak tyr 397
Pfak Tyr 397, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pfak tyr 397/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
pfak tyr 397 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
p fak tyr 397  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc p fak tyr 397
TGF-β signaling alters LCK and ZAP70 activation in murine CD4+ T cells. Murine CD4+ T cells isolated by negative selection were in vitro activated through TCR and CD28 costimulation for various time points with or without 10 ng/ml TGF-β. A, immunoblotting was performed on cell lysates for the phosphorylated and total protein LCK and ZAP-70 abundance. B and C, densitometry was performed across three independent experiments to quantitate the abundance of tyrosine phosphorylation for (B) LCK (Tyr-394) and (C) ZAP70 (Tyr-319). The abundance of phosphorylated protein was normalized to total protein. Shown are mean ± S.D.; p values were calculated by two-way ANOVA. Murine CD4+ T cells isolated by negative selection were in vitro activated through TCR and CD28 costimulation for 10 min with various doses of TGF-β. D, immunoblotting was performed on cell lysates for the phosphorylated and total protein abundance for LCK, ZAP-70, and <t>FAK.</t> E–H, densitometry was performed across three independent experiments to quantitate the abundance of tyrosine phosphorylation for (E) LCK (Tyr-394), (F) LCK (Tyr-505), (G) ZAP70 (Tyr-319), and (H) FAK <t>(Tyr-397).</t> The abundance of phosphorylated protein was normalized to total protein. Shown are mean ± S.D.; p values were calculated by one-way ANOVA with a Tukey multiple comparison test.
P Fak Tyr 397, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p fak tyr 397/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
p fak tyr 397 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier

Image Search Results


TGF-β signaling alters LCK and ZAP70 activation in murine CD4+ T cells. Murine CD4+ T cells isolated by negative selection were in vitro activated through TCR and CD28 costimulation for various time points with or without 10 ng/ml TGF-β. A, immunoblotting was performed on cell lysates for the phosphorylated and total protein LCK and ZAP-70 abundance. B and C, densitometry was performed across three independent experiments to quantitate the abundance of tyrosine phosphorylation for (B) LCK (Tyr-394) and (C) ZAP70 (Tyr-319). The abundance of phosphorylated protein was normalized to total protein. Shown are mean ± S.D.; p values were calculated by two-way ANOVA. Murine CD4+ T cells isolated by negative selection were in vitro activated through TCR and CD28 costimulation for 10 min with various doses of TGF-β. D, immunoblotting was performed on cell lysates for the phosphorylated and total protein abundance for LCK, ZAP-70, and FAK. E–H, densitometry was performed across three independent experiments to quantitate the abundance of tyrosine phosphorylation for (E) LCK (Tyr-394), (F) LCK (Tyr-505), (G) ZAP70 (Tyr-319), and (H) FAK (Tyr-397). The abundance of phosphorylated protein was normalized to total protein. Shown are mean ± S.D.; p values were calculated by one-way ANOVA with a Tukey multiple comparison test.

Journal: The Journal of Biological Chemistry

Article Title: Transforming growth factor β (TGF-β) receptor signaling regulates kinase networks and phosphatidylinositol metabolism during T-cell activation

doi: 10.1074/jbc.RA120.012572

Figure Lengend Snippet: TGF-β signaling alters LCK and ZAP70 activation in murine CD4+ T cells. Murine CD4+ T cells isolated by negative selection were in vitro activated through TCR and CD28 costimulation for various time points with or without 10 ng/ml TGF-β. A, immunoblotting was performed on cell lysates for the phosphorylated and total protein LCK and ZAP-70 abundance. B and C, densitometry was performed across three independent experiments to quantitate the abundance of tyrosine phosphorylation for (B) LCK (Tyr-394) and (C) ZAP70 (Tyr-319). The abundance of phosphorylated protein was normalized to total protein. Shown are mean ± S.D.; p values were calculated by two-way ANOVA. Murine CD4+ T cells isolated by negative selection were in vitro activated through TCR and CD28 costimulation for 10 min with various doses of TGF-β. D, immunoblotting was performed on cell lysates for the phosphorylated and total protein abundance for LCK, ZAP-70, and FAK. E–H, densitometry was performed across three independent experiments to quantitate the abundance of tyrosine phosphorylation for (E) LCK (Tyr-394), (F) LCK (Tyr-505), (G) ZAP70 (Tyr-319), and (H) FAK (Tyr-397). The abundance of phosphorylated protein was normalized to total protein. Shown are mean ± S.D.; p values were calculated by one-way ANOVA with a Tukey multiple comparison test.

Article Snippet: Antibodies used for Western blotting purchased from Cell Signaling Technology included: p-LCK (Tyr-505) (2751), LCK (Asp-88), p-tyrosine (P-Tyr-1000), p-P85 (Tyr-458), (E3U1H), P85 (19H8), P110 (D1Q7R), SMAD3 (C67H9), p-PKA (Thr-197) (D45D3), PKA (D38C6), CSK (C74C1), p-ZAP70 (Tyr-319) (65E4), ZAP70 (D1C10E), AKT (C67E7), p-AKT (Ser-473) (D9E), actin (13E5), FAK (D2R2E), and p-FAK (Tyr-397) (D20B1).

Techniques: Activation Assay, Isolation, Selection, In Vitro, Western Blot